Cardiff Blues contribute seven players to the starting line up, while six Ospreys also start alongside Luke Chateris of the Newport Gwent Dragons at the Millennium Stadium.
Sale scrum-half Dwayne Peel, who was not involved in Saturday's narrow loss to the All Blacks, is the only member of the squad to play his rugby outside the Magners League.
The Scarlets dominate the replacements bench, with four players from the West Wales region being joined by two from the Ospreys and one from the Blues.
Wales coach Warren Gatland makes five changes to his run-on side for the second of four autumn internationals as his team look to bounce back from last weekend's 19-12 defeat to Dan Carter and co.
Peel replaces Gareth Cooper in the No9 shirt, with Ospreys youngster Dan Biggar taking over from Stephen Jones at outside-half.
Tom James comes in for Shane Williams on the left wing in the third change to the backline.
Up front, Matthew Rees is replaced by Huw Bennett at hooker and Blues flanker Sam Warburton is named instead of regional team-mate Martyn Williams at openside.
Wales have lost three of their six encounters with Samoa, including humiliating defeats in both the 1991 and 1999 World Cups in Cardiff.
Unsurprisingly given the history between the two sides, Gatland is not expecting an easy ride against a Samoan squad that includes Glasgow's Magners League prop Justin Va'a.
"Samoa have proved to be a difficult side for Welsh teams in the past and we don't expect the standard to be any different on Friday night," said Gatland, whose squad face Argentina and then Australia later this month.
"We've named a strong side and shown the maximum amount of respect to Samoa.
"They are a tough physical team with players featuring for some of Europe's top sides and there is no question of us under-estimating what we are up against."
As for the changes in personnel following disappointment against New Zealand, Gatland says he has no qualms about the players he has brought in to face the Samoans.
"Dan Biggar is a bright prospect and his performances at the Ospreys this season mean he fully deserves his opportunity," added Gatland.
"Dan is surrounded by experience with Dwayne Peel and Jamie Roberts on either side of him which should help further smooth his transition to international rugby.
"Tom James has another chance to show us what he can do and Sam Warburton has been knocking on the door for some time after impressing the coaches on tour to North America this summer.
"But elsewhere we have maintained a consistency in selection and we will be looking to put a performance in on Friday night that will help create the momentum to finish off this series with wins and build on what we achieved for large parts of the game against New Zealand."
Wales vs Samoa, Friday, November 13, Millennium Stadium, kick-off 7.30pm
James Hook (Ospreys); Leigh Halfpenny, Tom Shanklin, Jamie Roberts, Tom James (all Cardiff Blues); Dan Biggar (Ospreys), Dwayne Peel (Sale); Gethin Jenkins (Blues), Huw Bennett, Paul James (both Ospreys), Luke Charteris (Newport Gwent Dragons), Alun-Wyn Jones (Ospreys), Andy Powell, Sam Warburton (both Blues), Ryan Jones (Ospreys, captain)
Replacements: Matthew Rees (Scarlets), Craig Mitchell (Ospreys), Bradley Davies (Blues), Jonathan Thomas (Ospreys), Martin Roberts, Jonathan Davies, Morgan Stoddart (all Scarlets)
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