The fixture is a repeat of last season's opener, when the Ospreys defeated Connacht 16-3 at the Sportsground, thanks to a try from Nikki Walker and 11 points from the boot of James Hook. The final 22 will be announced on Thursday at noon.
The squad for Friday's match has been confirmed as:
Forwards: Duncan Jones, Paul James, Cai Griffiths, Craig Mitchell, Richard Hibbard, Huw Bennett, Andy Lloyd, Ian Gough, Tom Smith, Jerry Collins, Marty Holah, Filo Tiatia, Ryan Jones
Backs: Barry Davies, Gareth Owen, Jonny Vaughton, Nikki Walker, Sonny Parker, Jonathan Spratt, Andrew Bishop, Dan Biggar, James Hook, Tom Isaacs, Mike Phillips