The full-back has been picked to start against Italian side Benetton Treviso with supporters handed the chance to acknowledge the contributions of the nation's record male cap holder and points' scorer before he hangs up his boots.
Paterson is one of six players named in the squad who will play their last game for the club on an evening that will double as an emotional farewell for a number of players.
The remaining five start the match on the bench and include the replacement front-row of Andrew Kelly, Kyle Traynor and Jack Gilding; back-row Alan MacDonald and stand-off Phil Godman, while the departing Mike Blair and Jim Thompson will miss the send-off due to injury (shoulder) and Scotland 7s duty respectively.
Season tickets holders can bring a friend to the Edinburgh Rugby match for free (using voucher C) while all fans can complete a great weekend of rugby with half-price party stand tickets for day two (Sunday) of the Emirates Airline Glasgow 7s using a code from the club's website edinburghrugby.org.
>> Click here to buy tickets for Edinburgh Rugby v Treviso
>> Click here to buy tickets for the Emirates Airline Glasgow 7s
Paterson said: "I've always said that I want to go out playing the best rugby I possibly can and I'm ready to move on to an exciting future.
"My retirement is tinged with sadness but my overriding and lasting feelings are ones of happiness, enjoyment and fulfilment, having achieved far more than I ever thought I would and could have in 14 years of the job.
"I'm delighted to be involved in this game and, as always, have done my best in training and will do my best in the game.
"We've had a great season in Europe and have a chance on Saturday to remedy our league form and finish the season well, both for Edinburgh Rugby and me personally."
Aside from emotional motivation, the club are looking to end the season with a trio of home wins, having defeated Scarlets in the RaboDirect PRO12 and Toulouse in the Heineken Cup quarter-final, leading into today's game last home game.
The importance of a winning farewell is galvanised by the players' desire to leave the club with their unbeaten home record against Italian teams intact.
Edinburgh Rugby team to play Treviso at Murrayfield Stadium on Saturday 5 May (kick-off 7.30pm): 15 Chris Paterson; 14 Tom Brown, 13 Nick De Luca, 12 Matt Scott, 11 Tim Visser; 10 Greig Laidlaw CAPTAIN, 9 Chris Leck; 1 Allan Jacobsen, 2 Ross Ford, 3 Geoff Cross, 4 Grant Gilchrist, 5 Sean Cox, 6 David Denton, 7 Ross Rennie, 8 Roddy Grant
Substitutes: 16 Andrew Kelly, 17 Kyle Traynor, 18 Jack Gilding, 19 Steven Turnbull, 20 Alan MacDonald, 21 Alex Black, 22 Harry Leonard, 23 Phil Godman
Not available through injury: Mike Blair (shoulder), Ben Cairns (knee), James King (groin), Steven Lawrie (knee), Esteban Lozada (chest)
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