The star-studded Welsh region were beaten 28-20 by Newport Gwent Dragons at Rodney Parade on Friday night.
That result saw the previous league leaders stay in fifth position in the standings, level on points with the sixth-placed Dragons and nine points adrift of table-topping Leinster.
But assistant coach Humphreys insists the Ospreys will get it right for the west Wales showdown with the Scarlets, the Heineken Cup quarter-final with Biarritz and a trio of tough Irish away fixtures in the Magners League over the next four weeks.
"We are taking absolutely nothing away from Dragons. I thought they were worthy winners. I thought they controlled the game marvelously," said Humphreys.
"They did everything we wanted to do out there. I thought Aled Brew's try was huge for them, the way they constructed it was outstanding.
"We were under no illusion about how difficult it would be here and are going away with our tail between our legs.
"We have two huge games coming up and these boys need to get back on the horse.
"The reaction in the changing room is the players are angry.
"They are proud people and it's not very nice to come to a place like this with such a vociferous crowd and be on the end of what was a very convincing victory for Dragons.
"We know what it takes to play well, we know what it takes to win the big games - and I have every faith these boys will do that."
Wales wing Shane Williams missed the derby with a minor shoulder problem but Humphreys predicted he would be fit for Friday's Magners clash with the Scarlets at the Liberty Stadium.
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